Test your Blood Group at home
Do you know your blood group. If you don’t know just follow simple 10 step and know what is your blood group.
Reagents & instrument required
1. Anti-Serum (anti-A, anti-B, anti-D)
2. Saline Water (NaCl)
3. Slide (Labeled A/B/D)
4. Needle
5. Cotton
6. Alcohol
[Now Its question where you get Reagents & instrument. If you are live in Dhaka you can collect Anti-Serum (anti-A, anti-B, anti-D) from Hathkhola & Elephant Road, and some popular Pharmacy in Shabag or Aziz Super Market. Saline Water (NaCl) solution can made in home using just salt & Pure Water.]
Procedure
1. Clean your hand in tap water,
2. Choose your finger where you collect your blood. [NB: You Can Use Any Part Of Your Body But Finger is easy to used]
3. Clean your finger with alcohol.
4. Which needle you used for penetration in your finger sterilize (জীবানু মুক্ত করণ) this.
5. Penetration in your finger & collect Blood
6. Place 1 drop of your blood in separated three slides.
7. Place 1 drop of your Saline water in separated three slides.
8. Place 1 drops of anti-A, 1 drop of anti-B, 1 drop of anti-D reagent separately on a labeled slide. Mix the cells and reagent using a clean stick. Spread each mixture evenly on the slide over an area of 10-15 mm diameter.
9. Tilt the slide and leave the test for 2 minutes at room temperature (22°-24°C). Then rock again and look for agglutination.
10. Record the results as shown as picture.
Result observation
1. When Antigen D slide Coagulation (জমাট বাধা) (Antigen & Antibody Reaction) then Blood group will Positive .other wise it’s negative.
2. When Antigen A slide Coagulation then Blood group will A group
3. When Antigen B slide Coagulation then Blood group will B group
4. When Antigen A & B slide Coagulation then Blood group will AB group
5. When Antigen A & B slide not Coagulation then Blood group will O group
[NB: Special Thanks for our Lab Attended (Pharmacy) Md Ashad who teach me how to test blood group]