Genetically Modified Tomato

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Offline Asif.Hossain

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Genetically Modified Tomato
« on: April 13, 2014, 10:43:55 AM »
A genetically modified tomato, or transgenic tomato is a tomato that has had its genes modified, using genetic engineering. The first commercially available genetically modified food was a tomato engineered to have a longer shelf life (the Flavr Savr). Currently there are no genetically modified tomatoes available commercially, but scientists are developing tomatoes with new traits like increased resistance to pests or environmental stresses. Other projects aim to enrich tomatoes with substances that may offer health benefits or be more nutritious. As well as aiming to produce novel crops, scientists produce genetically modified tomatoes to understand the function of genes naturally present in tomatoes.

The tomato originated from South America and was brought to Europe by the Spanish in the 16th century. Wild tomatoes are small, green and largely unappetizing, but after centuries of breeding there are now thousands of varieties grown worldwide. Agrobacterium-mediated genetic engineering techniques were developed in the late 1980s that could successfully transfer genetic material into the nuclear genome of tomatoes. Genetic material can also be inserted into a tomato cell's chloroplast and chromoplast pastimes using plastics. Tomatoes were the first food crop with an edible fruit where this was possible. Agrobacerium tumefaciens is an excellent species of soil dwelling bacteria that can infect plants with a piece of its own DNA. Agrobacterium mediated transformation is an effective and widely used approach to introduce foreign DNA into dicotyledon plants. The DNA gets a hold of the plant cell machinery and uses it to ensure the proliferation of the bacterial population. The advantage of this gene is that insecticidal toxin genes or other various herbicides can be engineered into the bacterial DNA. This bacterium shortens the plant breeding process. Most of all, it allows new genes to be engineered into crops.
Tomatoes have been used as a model organism to study the fruit ripening of climacteric fruit. To understand the mechanisms involved in the process of ripening, scientists have genetically engineered tomatoes.
In 1994, the Flavr Savr became the first commercially grown genetically engineered food to be granted a license for human consumption. A second copy of the tomato gene polygalacturonase was inserted into the tomato genome in the antisense direction. The polygalacturonase enzyme degrades pectin, a component of the tomato cell wall, causing the fruit to soften. When the antisense gene is expressed it interferes with the production of the polygalacturonase enzyme, delaying the ripening process. The Flavr Savr failed to achieve commercial success and was withdrawn from the market in 1997. Similar technology, but using a truncated version of the polygalacturonase gene, was used to make a tomato paste.
DNA Plant Technology (DNAP), Agritope and Monsanto developed tomatoes that delayed ripening by preventing the production of ethylene, a hormone that triggers ripening of fruit. All three tomatoes inhibited ethylene production by reducing the amount of 1-aminocyclopropane-1-carboxylic acid (ACC), the precursor to ethylene. DNAP's tomato, called Endless Summer, inserted a truncated version of the ACC synthase gene into the tomato that interfered with the endogenous ACC synthase.Monsanto's tomato was engineered with the ACC deaminase gene from the soil bacterium Pseudomonas chlororaphis that lowered ethylene levels by breaking down ACC.Agritope introduced an S-adenosylmethionine hydrolase (SAMase) encoding gene derived from the E. coli bacteriophage T3, which reduced the levels of S-adenosylmethionine, a precursor to ACC. Endless Summer was briefly tested in the marketplace, but patent arguments forced its withdrawal.
Scientists in India have delayed the ripening of tomatoes by silencing two genes encoding N-glycoprotein modifying enzymes, α-mannosidase and β-D-N-acetylhexosaminidase. The fruits produced were not visibly damaged after being stored at room temperature for 45 days, whereas unmodified tomatoes had gone rotten.In India, where 30% of fruit is wasted before it reaches the market due to a lack of refrigeration and poor road infrastructure, the researchers hope genetic engineering of the tomato may decrease wastage.
Abiotic stresses like frost, drought and increased salinity are a limiting factor to the growth of tomatoes.While no genetically modified stress tolerant plants are currently commercialised, transgenic approaches have been researched. An early tomato was developed that contained an antifreeze gene (afa3) from the winter flounder with the aim of increasing the tomato's tolerance to frost (see Fish tomato). The antifreeze protein was found to inhibit ice recrystallization in the flounders blood, but had no effect when expressed in transgenic tobacco.The resulting tomato was never commercialized, but raised ethical questions over adding genes from one kingdom to another.
Other genes from various species have been inserted into the tomato with the hope of increasing their resistance to various environmental factors. A gene from rice (Osmyb4), which codes for a transcription factor, that was shown to increase cold and drought tolerance in transgenic Arabidopsis thaliana plants was inserted into the tomato. This resulted in increased drought tolerance, but did not appear to have any effect on cold tolerance. Overexpressing a vacuolar Na+/H+ antiport (AtNHX1) from A. thaliana lead to salt accumulating in the leaves of the plants, but not in the fruit and allowed them to grow more in salt solutions than wildtype plants.They were the first salt-tolerant, edible plants ever created. Tobacco osmotic genes overexpressed in tomatoes produced plants that held a higher water content than wildtype plants increasing tolerance to drought and salt stress.
The insecticidal toxin from the bacterium Bacillus thuringiensis has been inserted into a tomato plant. When field tested they showed resistance to the tobacco hornworm (Manduca sexta), tomato fruitworm (Heliothis zea), the tomato pinworm (Keiferia lycopersicella) and the tomato fruit borer (Helicoverpa armigera).A 91 day feeding trail in rats showed no adverse effects, but the Bt tomato has never been commercialised. Tomatoes resistant to a root knot nematode have been created by inserting a cysteine proteinase inhibitor gene from taro. A chemically synthesised ceropin B gene, usually found in the giant silk moth (Hyalophora cecropia), has been introduced into tomato plants and in vivo studies show significant resistance to bacterial wilt and bacterial spot.When the cell wall proteins, polygalacturonase and expansin are prevented from being produced in fruits, they are less susceptible to the fungus Botrytis cinerea than normal tomatoes. Pest resistant tomatoes can reduce the ecological footprint of tomato production while at the same time increase farm income.
Tomatoes have been altered in attempts to improve their flavour or nutritional content. In 2000, the concentration of pro-vitamin A was increased by adding a bacterial gene encoding phytoene desaturase, although the total amount of carotenoids remained equal.The researchers admitted at the time that it had no prospect of being grown commercially due to the anti-GM climate. Sue Meyer of the pressure group Genewatch, told The Independent that she believed, "If you change the basic biochemistry, you could alter the levels of other nutrients very important for health".More recently, scientists have increased the production of anthocyanin, an antioxidant in tomatoes in several ways. One group added a transcription factor for the production of anthocyanin from Arabidopsis thaliana[35] whereas another used transcription factors from snapdragon (Antirrhinum).[36] When the snapdragon genes where used, the fruits had similar anthocyanin concentrations to blackberries and blueberries, and when fed to cancer susceptible mice, extended their life span. Another group has tried to increase the levels of isoflavone, known for its potential cancer preventative properties, by introducing the soybean isoflavone synthase into tomatoes.
When geraniol synthase from lemon basil (Ocimum basilicum) was expressed in tomato fruits under a fruit-specific promoter, 60% of untrained taste testers preferred the taste and smell of the transgenic tomatoes. The fruits contained around half the amount of lycopene, reducing the health benefits of eating them.
Tomatoes (along with potatoes, bananas and other plants) are being investigated as vehicles for delivering edible vaccines. Clinical trials have been conducted on mice using tomatoes expressing antibodies or proteins that stimulate antibody production targeted to norovirus, hepatitis B, rabies, HIV, anthrax and respiratory syncytial virus. Korean scientists are looking at using the tomato to expressing a vaccine against Alzheimer's disease. Hilary Koprowski, who was involved in the development of the polio vaccine, is leading a group of researchers in developing a tomato expressing a recombinant vaccine to SARS.
Tomatoes are used as a model organism in scientific research and they are frequently genetically modified to further our understanding of particular processes. Tomatoes have been used as a model in map-based cloning, where trangsenic plants must be created to prove that a gene has been successfully isolated. The plant peptide hormone, systemin was first identified in tomato plants and genetic modification has been used to demonstrate its function, by adding antisense genes to silence the native gene, or by adding extra copies of the native gene

Muhammad Asif Hossain
Coordination Officer
Department of Pharmacy
Daffodil International University

Offline naser.te

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Re: Genetically Modified Tomato
« Reply #1 on: August 12, 2016, 01:53:21 AM »
New information.
Abu Naser Md. Ahsanul Haque
Assistant Professor