Scientists previously described how miRNA transfers genetic regulatory information from cell to cell within protective nano-vesicles (sacs) known as exosomes. In this study, the Yale team — led by professor of medicine (immunology) Philip Askenase in collaboration with visiting professor Krzysztof Bryniarski from Jagiellonian University in Krakow Poland — examined how miRNA from mouse immune T cells are delivered independently of these vesicles.
Although such “free” extracellular RNA, or exRNA, is the dominant form of RNA in the circulation, the function of exRNA was not known. However, the researchers discovered that exRNA can associate with exosomes from companion cells of the targeted cells (in this case, companion immune system B cells) to transfer the genetic messages via specific structures (antigens) on the final targeted cell.
The findings are significant, say the researchers, because they show not only how freely circulating miRNA transfers between cells, but also how it can influence the function of targeted cells in an antigen-specific way. The research provides a model for future study of miRNA information transfer between cells, and as a basis for the potential development of unique RNA genetic therapies for human diseases, including allergy, autoimmunity, and even cancer.
Other authors include, Wlodzimierz Ptak, Katarzyna Nazimek, Emilia Martin, Marian Szczepanik, and Marek Sanak.
Publication: Krzysztof Bryniarski, et al., “Free Extracellular miRNA Functionally Targets Cells by Transfecting Exosomes from Their Companion Cells,” PLOS One, 2015; DOI: 10.1371/journal.pone.0122991
Source: Ziba Kashef, Yale University