Daffodil International University

Faculty of Allied Health Sciences => Pharmacy => Topic started by: Hello Obayed on April 22, 2015, 12:26:09 PM

Title: Recombinant Protein Production of Human Immune Protein
Post by: Hello Obayed on April 22, 2015, 12:26:09 PM

Recombinant production of functional full-length and truncated human TRAM/TICAM-2 adaptor protein involved in Toll-like receptor and interferon signaling


Highlights

 Full-length and truncated TRAM proteins were successfully expressed in E. coli and purified.
Systematic construct design and small-scale expression helped to determine the boundaries of the TRAM TIR domain.
 TRAM and TLR4 TIR domains interacts physically; the BB loop regions of both proteins are crucial for this interaction.
 Large-scale purification and biophysical characterization identified a TRAM construct suitable for structural studies.

For Abstract/Full paper, please wirte to me
Obayed

Title: Re: Recombinant Protein Production of Human Immune Protein
Post by: imran986 on July 07, 2015, 11:58:58 AM

Nice